Protein Info for PP_5535 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 152 transmembrane" amino acids 13 to 35 (23 residues), see Phobius details amino acids 40 to 67 (28 residues), see Phobius details amino acids 80 to 110 (31 residues), see Phobius details amino acids 129 to 150 (22 residues), see Phobius details PF11911: DUF3429" amino acids 10 to 145 (136 residues), 123.2 bits, see alignment E=5.1e-40

Best Hits

KEGG orthology group: None (inferred from 93% identity to ppf:Pput_3206)

Predicted SEED Role

"uncharacterized conserved membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A140FW89 at UniProt or InterPro

Protein Sequence (152 amino acids)

>PP_5535 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MSSVATALSPSRFTVLGYGGLLPFIGLTLLILFWAEYRAFFSMMLVGYGAVILSFVGALH
WGFAMTLQDLPAKQRQERLVWSVIPALIGWVGTLLPVPVGCLVLTSGFVGHLWQDRKLAQ
AVPGWYLPMRVHLTAVASLCLLVSALAVSIHF