Protein Info for PP_5413 in Pseudomonas putida KT2440

Annotation: ATP synthase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 TIGR01039: ATP synthase F1, beta subunit" amino acids 3 to 458 (456 residues), 839.1 bits, see alignment E=4.4e-257 PF02874: ATP-synt_ab_N" amino acids 6 to 71 (66 residues), 69.9 bits, see alignment E=3.3e-23 PF00006: ATP-synt_ab" amino acids 128 to 340 (213 residues), 241.8 bits, see alignment E=9.3e-76 PF22919: ATP-synt_VA_C" amino acids 345 to 422 (78 residues), 55.6 bits, see alignment E=6.9e-19

Best Hits

Swiss-Prot: 100% identical to ATPB_PSEPK: ATP synthase subunit beta (atpD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02112, F-type H+-transporting ATPase subunit beta [EC: 3.6.3.14] (inferred from 100% identity to ppg:PputGB1_5431)

MetaCyc: 84% identical to ATP synthase F1 complex subunit beta (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP synthase beta chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88BX4 at UniProt or InterPro

Protein Sequence (458 amino acids)

>PP_5413 ATP synthase subunit beta (Pseudomonas putida KT2440)
MSSGRIVQIIGAVIDVEFPRDVVPSVYNALKVQGAETTLEVQQQLGDGVVRTIAMGSTEG
LKRGLDVVDTGAAISVPVGKATLGRIMDVLGNPIDEAGPIGEEERRGIHQPAPSFADQAG
GNDLLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMMELIRNIAMEHSGYSVFAGVGE
RTREGNDFYHEMKDSNVLDKVALVYGQMNEPPGNRLRVALTGLTMAEKFRDEGNDVLLFV
DNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVLQERITSTKEGSITSVQAVYVPAD
DLTDPSPATTFAHLDATVVLSRDIASLGIYPAVDPLDSTSRQLDPNVIGNEHYETARGVQ
YVLQRYKELKDIIAILGMDELSEADKQLVARARKIQRFLSQPFFVAEVFTGSPGKYVSLK
DTIAGFSGILKGDYDHLPEQAFYMVGSIDEAIEKAKKL