Protein Info for PP_5374 in Pseudomonas putida KT2440

Annotation: Choline/carnitine/betaine transporter family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 54 to 72 (19 residues), see Phobius details amino acids 92 to 113 (22 residues), see Phobius details amino acids 146 to 164 (19 residues), see Phobius details amino acids 184 to 206 (23 residues), see Phobius details amino acids 227 to 251 (25 residues), see Phobius details amino acids 263 to 283 (21 residues), see Phobius details amino acids 318 to 336 (19 residues), see Phobius details amino acids 347 to 368 (22 residues), see Phobius details amino acids 426 to 446 (21 residues), see Phobius details amino acids 468 to 487 (20 residues), see Phobius details amino acids 493 to 514 (22 residues), see Phobius details PF02028: BCCT" amino acids 15 to 521 (507 residues), 588.9 bits, see alignment E=3.4e-181 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 54 to 521 (468 residues), 459.1 bits, see alignment E=7.5e-142

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5374)

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C09 at UniProt or InterPro

Protein Sequence (567 amino acids)

>PP_5374 Choline/carnitine/betaine transporter family protein (Pseudomonas putida KT2440)
MRAESGLLKGLNPTVTIWSLLAVVAFVLFCALFAEHAAAVFQRASDFILQNFKWFYLISV
TGVLGLLIYLMCSKFGSMKLGRDDDKPEFSFGSWIAMLFSGGMGIGLIFWSVAEPMWHYA
GNPFATGLTDEAATTAMRITLFHWGLHPWAIFTIVGLGLAYFAYRKGLPLSMRSILYPLI
GERIYGPIGHVVDILAVVITAFGVSQSLGLGVVQMNTGLSQVFDLPISLGVQITLIVLIT
LVTTVSVMAGVSRGMKRLSEWNMLLSVVLVVLILLLGPTRYIINLMLESTGDYASHVVGL
SFWSDTQKDSGWQNWWTAFYWPWWMTWGPFVGLFIARISKGRSIRELIAGALLVPTLVTI
IWMSVFGGTALKAEQLDRQQHAELVASGELQGDKAKYEGGTVLLATKQETTAAMFTLLHK
IDAGTLGKVLSVLVCILLATYFITSADSGTQVLCTLNSMGSANPPQSIRLLWCILEGAIA
MALLMAGGLKAIQMASIAAGLPIAGFILLISYTLMRSLRQEMADGLLDTPPGGRLPIPGI
HPQGITPVAGPLACEGLDMGLTAKKAS