Protein Info for PP_5328 in Pseudomonas putida KT2440

Annotation: putative phosphate transport system permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 762 transmembrane" amino acids 40 to 66 (27 residues), see Phobius details amino acids 466 to 488 (23 residues), see Phobius details amino acids 501 to 524 (24 residues), see Phobius details amino acids 530 to 551 (22 residues), see Phobius details amino acids 564 to 582 (19 residues), see Phobius details amino acids 613 to 632 (20 residues), see Phobius details amino acids 661 to 682 (22 residues), see Phobius details amino acids 727 to 748 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 479 to 757 (279 residues), 29.7 bits, see alignment E=2.6e-11

Best Hits

KEGG orthology group: K02037, phosphate transport system permease protein (inferred from 100% identity to ppf:Pput_5236)

Predicted SEED Role

"Phosphate transport system permease protein PstC (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88C55 at UniProt or InterPro

Protein Sequence (762 amino acids)

>PP_5328 putative phosphate transport system permease protein (Pseudomonas putida KT2440)
MNDLANSNMTQNSQPVRIDFNTPELQRKRRMRALKDRLTRWYVLVGGLAVLAAITLIFFY
LAYVVLPLFQGAELTSKKALEPTWLQQDAGKPLMIALEEQNQVGMRVSDKGQALFFDTKT
GNELKRVELPVPADTQVTSISTDQPGSALVVLGLSNGQALVFQHTYKITYPDNKKTISPG
IDYPYGEQSFVLDEQGRALDHVSVNVNGDTLLLAGSTGAHLQVVELTRTENMMTEEVTTE
QNRIELPQMTETVKNIFIDPRQHWLYVINGRATADVFSLRDKSLNGRYKLSDSADTEITA
TAQLVGGISLIIGDSKGGLAQWFMARDPDGESRFKQIRTFQMGKAPIVQIDAEERRKGFI
ALDAEGKLGVFHSTAHRTLLVEPAAEGPGTLALSPRANRIIIEEGGKLLPLSLRNPHPEV
SFSALWGKVWYENYDEPKYVWQSTASNTDFEPKLSLSPLTFGTLKAAFYAMILAAPLAIA
AAIYTAYFMAPRMRRKVKPVIELMEAMPTVILGFFAGLFLAPYLEGHLPGVFSLFLLMPI
GILLAGFFWSRLPESIRLRVPDGWEAAILIPVILFTGWFALTMSPHLESWFFGGDMRLWI
TNDLGITYDQRNALVVGIAMGFAVIPNIYSIAEDAVFSVPRSLTLGSLALGATPWQTLTR
VVILTASPGIFSALMIGMGRAVGETMIVLMATGNTPVMEMNLFEGMRTLAANVAVEMPES
EVGGSHYRVLFLAALVLLMFTFIMNTLAELIRQRLRKKYSSL