Protein Info for PP_5286 in Pseudomonas putida KT2440
Annotation: deoxyuridine 5'-triphosphate nucleotidohydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to DUT_PSEPK: Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K01520, dUTP pyrophosphatase [EC: 3.6.1.23] (inferred from 98% identity to ppf:Pput_5196)MetaCyc: 70% identical to dUTP diphosphatase (Escherichia coli K-12 substr. MG1655)
dUTP diphosphatase. [EC: 3.6.1.23, 3.6.1.9]
Predicted SEED Role
"Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23)" in subsystem Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.23)
MetaCyc Pathways
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
- dZTP biosynthesis (3/5 steps found)
- pyrimidine deoxyribonucleotides dephosphorylation (1/3 steps found)
- tunicamycin biosynthesis (2/9 steps found)
KEGG Metabolic Maps
- Nicotinate and nicotinamide metabolism
- Pantothenate and CoA biosynthesis
- Purine metabolism
- Pyrimidine metabolism
- Riboflavin metabolism
- Starch and sucrose metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 3.6.1.23 or 3.6.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88C95 at UniProt or InterPro
Protein Sequence (151 amino acids)
>PP_5286 deoxyuridine 5'-triphosphate nucleotidohydrolase (Pseudomonas putida KT2440) MHALQAKILDPRLGSEFPLPAYATPGSAGLDLRALLKEDTILEPGQTVLIPTGLSIYIGD PGLAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSCWNRGNTPFTIAVGERIAQLV LVPVVQAHFEIVEAFDESQRGAGGFGHSGSH