Protein Info for PP_5171 in Pseudomonas putida KT2440

Annotation: sulfate ABC transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 24 to 338 (315 residues), 444.9 bits, see alignment E=8e-138 PF01547: SBP_bac_1" amino acids 42 to 284 (243 residues), 67.9 bits, see alignment E=1.6e-22 PF13531: SBP_bac_11" amino acids 51 to 292 (242 residues), 128.3 bits, see alignment E=4e-41

Best Hits

Swiss-Prot: 65% identical to SUBI_SHIFL: Sulfate-binding protein (sbp) from Shigella flexneri

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to ppu:PP_5171)

MetaCyc: 65% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CK9 at UniProt or InterPro

Protein Sequence (342 amino acids)

>PP_5171 sulfate ABC transporter (Pseudomonas putida KT2440)
MPIGSFTMSIRRYALAALASAVFAGSAIAKDYELLNVSYDPTRELYQQYNAEFIKHWQQA
HPGDKVKIQQSHGGSGKQARAVIDGLRADVVTLALAGDIDEVAKLGKTLPENWQTRLPDA
STPYTSTIVFLVRKGNPKGIKDWGDLIKKDVSVITPNPKTSGGARWNFLAAWAYGLKVGG
SEEKAKAYVQELFKHVPVLDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGANKFE
IVVPSLSILAEPPVAVVDKNAEKKGNTEIAIEYLKHLYSPAGQKIAAENFYRPRDEKVAA
QFGKQFPKLDLVTIDKDFGGWKTAQPKFFNDGGVFDQIYQAQ