Protein Info for PP_5154 in Pseudomonas putida KT2440

Annotation: putative Oxidoreductase, FAD-binding

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 PF01565: FAD_binding_4" amino acids 31 to 169 (139 residues), 129.9 bits, see alignment E=5.5e-42 PF02913: FAD-oxidase_C" amino acids 207 to 451 (245 residues), 212 bits, see alignment E=1.2e-66

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5154)

Predicted SEED Role

"D-2-hydroxyglutarate dehydrogenase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CM6 at UniProt or InterPro

Protein Sequence (455 amino acids)

>PP_5154 putative Oxidoreductase, FAD-binding (Pseudomonas putida KT2440)
MTLVDPGKVLTDPVSLEAYGKDWTKHYPPAPSAIVFPKTIEQVQAIVHWANAHKVALVPS
GGRTGLSAGAVAANGEVVVAFDYMNQILGFNAFDRTVVCQPGVVTRQLQTYAEEQGLYYP
VDFASSGSSQIGGNIGTNAGGIKVIRYGMTRNWVAGLKVVTGKGELLELNKDLIKNATGY
DLRQLFIGAEGTLGFVVEATMRLDRAPRNLTAMVLGTPDFDSIMPVLHAFQGKLDLTAFE
FFSDKGLAKILARGDVPAPFATDCPFYALLEFEASTEDVANEALATFEHCVEQGWVLDGV
MSQSESQLKNLWKLREYLSETISHWTPYKNDISVTVSKVPAFLRDIDAIVSEHYPDYEVV
WYGHIGDGNLHLNILKPEHMSKDDFFASCAKVNKWVFEIVERYNGSISAEHGVGMTKRDY
LGYSRSPEEIACMKAIKAVFDPNGIMNPGKIFAPE