Protein Info for PP_5139 in Pseudomonas putida KT2440

Annotation: cadmium translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 transmembrane" amino acids 136 to 155 (20 residues), see Phobius details amino acids 161 to 179 (19 residues), see Phobius details amino acids 191 to 207 (17 residues), see Phobius details amino acids 213 to 231 (19 residues), see Phobius details amino acids 364 to 389 (26 residues), see Phobius details amino acids 395 to 421 (27 residues), see Phobius details amino acids 699 to 720 (22 residues), see Phobius details amino acids 726 to 747 (22 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 193 to 747 (555 residues), 584.5 bits, see alignment E=5.4e-179 TIGR01494: HAD ATPase, P-type, family IC" amino acids 216 to 730 (515 residues), 258.4 bits, see alignment E=2.1e-80 PF00122: E1-E2_ATPase" amino acids 244 to 345 (102 residues), 100.9 bits, see alignment E=4.6e-33 PF00702: Hydrolase" amino acids 444 to 657 (214 residues), 122.6 bits, see alignment E=4.2e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to ppu:PP_5139)

MetaCyc: 100% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CP1 at UniProt or InterPro

Protein Sequence (750 amino acids)

>PP_5139 cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
MNQPVSHEHKHPHDHAHGDDDHGHAAHGHSCCGAKAAPPLVQLSETASAQAQLSRFRIEA
MDCPTEQTLIQDKLSKLAGIEQLEFNLINRVLGVRHTLDGTADIERAIDSLGMKAEPIAA
QDDGSASVPQPAKAHWWPLALSGVAAIAAEIVHFAALAPEWVVAGLALAAILGCGLGTYK
KGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMVLFTVAELIEARSLDRARNAIGG
LMQLTPDMATVQQADGQWRELDVREVAIGALVRVRPGERIGLDGEVTRGQSSVDQAPITG
ESLPVEKGVGDKLFAGTINQAGALEFRVTAAAGQSTLARIIKAVEEAQGARAPTQRFVDR
FSRIYTPVVFAIALAVALIPPLFLAGAWFDWVYRALVLLVVACPCALVISTPVTIVSGLA
AAARKGILIKGGVYLEGGRHLDFLALDKTGTITHGKPVQTDAKVLAPLFEDRAQALAASL
GERSDHPVSRAIAEFGKQQGLALSEVSDFVALAGRGVRGVIAGEVYHLGNHRLVEELGLC
SPALEAQLDALERQGKTVVLLLDRSGPLALFAVADTVKDSSRQAIAELHQLGIKTVMLTG
DNPHTAQAIAAVVGIDRAEGNLLPADKLKTIEALYAQGHRVGMVGDGINDAPALARAEIG
FAMAAAGTDTAIETADVALMDDDLRKIPAFVRLSRQSAAILMQNIVLALGIKAIFLAITF
AGMATMWMAVFADMGVSLLVVFNGLRLLRK