Protein Info for PP_5127 in Pseudomonas putida KT2440

Annotation: ribosomal RNA large subunit methyltransferase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 PF17785: PUA_3" amino acids 6 to 72 (67 residues), 51.5 bits, see alignment E=3.1e-17 PF02475: Met_10" amino acids 123 to 288 (166 residues), 37.4 bits, see alignment E=1.1e-12 PF10672: Methyltrans_SAM" amino acids 172 to 340 (169 residues), 64.2 bits, see alignment E=5.3e-21 PF03602: Cons_hypoth95" amino acids 215 to 341 (127 residues), 41.4 bits, see alignment E=5.9e-14 PF13847: Methyltransf_31" amino acids 220 to 340 (121 residues), 29.7 bits, see alignment E=2.3e-10 PF05175: MTS" amino acids 220 to 335 (116 residues), 32.9 bits, see alignment E=2.3e-11 PF09445: Methyltransf_15" amino acids 222 to 349 (128 residues), 38.4 bits, see alignment E=4.5e-13 PF01170: UPF0020" amino acids 245 to 340 (96 residues), 23.6 bits, see alignment E=1.7e-08

Best Hits

Swiss-Prot: 37% identical to RLMI_ERWT9: Ribosomal RNA large subunit methyltransferase I (rlmI) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 100% identity to ppg:PputGB1_5177)

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CQ3 at UniProt or InterPro

Protein Sequence (398 amino acids)

>PP_5127 ribosomal RNA large subunit methyltransferase I (Pseudomonas putida KT2440)
MSLPSLRLKANADRRLRAGHLWVYSNEVDVSATPLQGFQAGQQAILEAANGKPLGIVALS
PNNLICARLLSRDIKLPLDKSLLVHRLNVALSLRERLFEQPCYRLVYGDSDLLPGLVVDR
FFDILVVQLASATMEAHKDDVIAALVQVLKPSGILFKNDSAARDAEGLQRYVETVYGEVP
DWVPLEENGVKFEAPVREGQKTGWFYDHRMNRARLAPYVKGKRVLDLFSYIGGWGVQAGA
FGASEVFCVDASGFALDGVERNAALNGISEKLTCIEGDVFEALRELKAAEERFDVIIADP
PAFIKRKKDLKNGEAAYRRLNEQAMRMLNKDGILVSASCSMHLPEDDLNNILLTSARHLD
RNMQLLERGGQGPDHPVHPAIAETRYIKSITCRLLPNS