Protein Info for PP_5115 in Pseudomonas putida KT2440

Annotation: putative Zinc protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 PF00675: Peptidase_M16" amino acids 25 to 156 (132 residues), 84.4 bits, see alignment E=8e-28 PF05193: Peptidase_M16_C" amino acids 178 to 361 (184 residues), 82.4 bits, see alignment E=3.9e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_5115)

Predicted SEED Role

"FIG146397: peptidase, M16 family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CR5 at UniProt or InterPro

Protein Sequence (433 amino acids)

>PP_5115 putative Zinc protease (Pseudomonas putida KT2440)
MAEHPSPPLMAASKGSFTLANGLHVCLREDHRAPLVSVQLWYHVGSSYEPEGHTGLSHAL
EHLLFEGSSKLAPGQYSTLMTRLGGDPNAFTYADATVFPLTLPTRHLEIALEAMADVMAS
ATLGDTPFARELAVVMAERREAVDNNPWALALEHHDLLAYGNSGHGTPVIGHMSDLESLT
PAAARTWYKTWYHPNNATLAVAGDISLLQLQTLVTRHFAAIPAHRLPMRPLPTGPSSQGR
RFQTLRLPGLHNGVIISFKLPSQRTAQSARQAYALRLLPDLLANGYSSILQRRLLLEEPI
LQYIKATYEPWQRGDSLLTLYAFCSPNVTPQTAAERLVQEIEAFRQSAPAKEHLQRAKAR
LLARLLFERDDIAEQAQTMGKQAACGLPVISLEEEQQAIETVTAEQVGLAAYEFLTHSRA
AFTFTHHKESAHD