Protein Info for PP_5103 in Pseudomonas putida KT2440

Annotation: tRNA (guanine-N(7)-)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 240 TIGR00091: tRNA (guanine-N(7)-)-methyltransferase" amino acids 52 to 239 (188 residues), 202.4 bits, see alignment E=2.3e-64 PF02390: Methyltransf_4" amino acids 65 to 234 (170 residues), 186.6 bits, see alignment E=1.4e-59

Best Hits

Swiss-Prot: 100% identical to TRMB_PSEPG: tRNA (guanine-N(7)-)-methyltransferase (trmB) from Pseudomonas putida (strain GB-1)

KEGG orthology group: K03439, tRNA (guanine-N7-)-methyltransferase [EC: 2.1.1.33] (inferred from 100% identity to ppf:Pput_4976)

MetaCyc: 61% identical to tRNA m7G46 methyltransferase (Escherichia coli K-12 substr. MG1655)
tRNA (guanine-N(7)-)-methyltransferase. [EC: 2.1.1.33]

Predicted SEED Role

"tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)" (EC 2.1.1.33)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CS7 at UniProt or InterPro

Protein Sequence (240 amino acids)

>PP_5103 tRNA (guanine-N(7)-)-methyltransferase (Pseudomonas putida KT2440)
MTESHDTPITPDGEARPHRRIKSFVMRAGRMTEGQQRGLDQGGPLYILPLADSPVDYDQV
FGRSAPRTLEIGFGMGHSLLEMAAAAPEQDFIGVEVHRPGVGALLNGVLTQGLKNLRVYD
CDAIEVLNRCVADNSLDRLMLFFPDPWHKARHHKRRIVQLEFAELVRRKLKPGGVFHMAT
DWEPYAEYMLEVMSAAPGYRNRAADGTYVPRPEERPITKFERRGERLGHGVWDLKFEKVD