Protein Info for PP_5096 in Pseudomonas putida KT2440

Annotation: putative factor involved in shape determination

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 196 transmembrane" amino acids 7 to 29 (23 residues), see Phobius details amino acids 70 to 91 (22 residues), see Phobius details amino acids 99 to 127 (29 residues), see Phobius details amino acids 165 to 186 (22 residues), see Phobius details PF02325: YGGT" amino acids 14 to 80 (67 residues), 67.6 bits, see alignment E=5.1e-23 amino acids 109 to 178 (70 residues), 72.4 bits, see alignment E=1.6e-24

Best Hits

Swiss-Prot: 61% identical to Y392_PSEAE: Uncharacterized protein PA0392 (PA0392) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02221, YggT family protein (inferred from 98% identity to ppw:PputW619_0369)

Predicted SEED Role

"Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock)" in subsystem Phosphate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CT4 at UniProt or InterPro

Protein Sequence (196 amino acids)

>PP_5096 putative factor involved in shape determination (Pseudomonas putida KT2440)
MNALSGAAIFVVQTLVSLYLVIVLLRFVLQLVKANFYNPLCQFAVRATQPLLKPIRRIIP
SVGGLDTSSLLLAVVIQALLMGFVLMVTYGTFGDVLHLLMWAIIGITSLFLKIFWVAMIV
MVIVSWVAPNSHNPAAELAYQISEPVLAPFRRLVPNLGGMDISPIFAFLAIQVVQSFVMP
PLAAYAGMPQELWRMI