Protein Info for PP_5083 in Pseudomonas putida KT2440

Annotation: putative Type IV pili biogenesis protein PilM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 PF11104: PilM_2" amino acids 14 to 176 (163 residues), 103.7 bits, see alignment E=5.1e-34

Best Hits

KEGG orthology group: K02662, type IV pilus assembly protein PilM (inferred from 100% identity to ppu:PP_5083)

Predicted SEED Role

"Type IV pilus biogenesis protein PilM" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CU7 at UniProt or InterPro

Protein Sequence (293 amino acids)

>PP_5083 putative Type IV pili biogenesis protein PilM (Pseudomonas putida KT2440)
MFGRFGKDAGSLVGVEITPDAVRIMQLQQRNRRCRVMGSAQELFQAPLADDWAQAPGVVV
AALQRAYRRSGLRQRRVALALPASQVICKLCHLPVEQSGAQLEAQLLADAERLFPFPLED
LALDFQVMGASHDQPGCSEVMVAACRQSALASLEAVVEEAGLQLEAVEVDNIALCRMLPQ
GGLEGAALLRVENRSVTLHDWQPGRAYQRCELQTTGPDMNGQWPERLQALLVGQSRPAGL
WVASSSSINPGWLQALGSRLNVPCTYLPGLAGLEHVDGAMLLACALALGGLRP