Protein Info for PP_5080 in Pseudomonas putida KT2440

Annotation: type 4 pili biogenesis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 412 signal peptide" amino acids 1 to 13 (13 residues), see Phobius details PF07660: STN" amino acids 43 to 90 (48 residues), 39.3 bits, see alignment 6.5e-14 PF03958: Secretin_N" amino acids 116 to 180 (65 residues), 52.1 bits, see alignment E=9.3e-18 PF00263: Secretin" amino acids 247 to 403 (157 residues), 171.3 bits, see alignment E=2.2e-54

Best Hits

KEGG orthology group: K02666, type IV pilus assembly protein PilQ (inferred from 100% identity to ppu:PP_5080)

Predicted SEED Role

"Type IV pilus biogenesis protein PilQ" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CV0 at UniProt or InterPro

Protein Sequence (412 amino acids)

>PP_5080 type 4 pili biogenesis protein (Pseudomonas putida KT2440)
MATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQD
VPWDQALDLVLRSKGLSRRQEGNVLLVAPAAEFAAQSADARVSQALDAHLQPLRRELLPI
HHAKAADLAQLLLAGLEGDGIPPASLSVDERTNTLVVHLPADRLAEMRQLVAQLDVPVRQ
VAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGLGLLRGDVL
LDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSL
EVTPQITPDNKVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYST
SQNNVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAIAVSR