Protein Info for PP_5076 in Pseudomonas putida KT2440
Annotation: L-glutamate synthase(NADPH) alpha subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to GLTB_HALED: Glutamate synthase [NADPH] large chain (gltB) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 69% identity to abo:ABO_2229)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88CV4 at UniProt or InterPro
Protein Sequence (1481 amino acids)
>PP_5076 L-glutamate synthase(NADPH) alpha subunit (Pseudomonas putida KT2440) MKTGLYHPEEFKDNCGFGLIAHMTGEPSHHLLQTAMQALTCMTHRGGINADGKTGDGCGL LMQKPDQFLRAMAQEHFAVELPKQYAVGMVFFNQDPVKAEAARANMDREILAAGLKLVGW RKVPIDTSVLGRLALERLPQIEQVFIGGEGLSDQEFAIKLFSARRRSSVANAHDADHYIC SFSHKTIIYKGLMMPRDLAAFYPDLGDERLQTAICVFHQRFSTNTLPKWPLAQPFRFLAH NGEINTITGNRNWAVARRTKFANDQIPDLEELGPLVNRVGSDSSSMDNMLELMVTGGIDL FRGVRMLVPPAWQNVETMDADLRAFYEYNSMHMEPWDGPAGIVMTEGRHAVCLLDRNGLR PARWVTTTNGYITIASEIGVWGYQPEEVLAKGRVGPGQILAVDTETGQILDTDAIDNRLK SRHPYKRWLRQHATRIQATLTDDQGVASYDADQLKQYMKMFQVTFEERDQVLRPLGEQGQ EAVGSMGDDTPMAVLSQRVRSPYDFFRQQFAQVTNPPIDPLREAIVMSLEICLGAERNIF QESPEHASRVILSSPVISPAKWRSLMNLEREGFDRQLIDLNYEQSVGLEAAIRNIADQAE EAVRGGKTQLVLSDRYIAPGKLPVHASLAVGAVHHRLTEQGLRCDSNILVETATARDPHH FAVLLGFGASAVYPYLAYEVLADLIRTGEVLGDLDEVFKYYRKGISKGLLKILSKMGIST IASYRGAQLFEAIGLAEEVVGLSFKGVSSRIKGARFEDLENDQKLLAAEAWSARKPIQQG GLLKFVHGGEYHAYNPDVVNTLQAAVQQGDYAKFKEYTTLVDQRPVSMIRDLLKVKVADQ PLALEQVEPLEAILKRFDSAGISLGALSPEAHEALAEAMNRLGARSNSGEGGEDPSRYGT IKSSKIKQVATGRFGVTPEYLVNAEVLQIKVAQGAKPGEGGQLPGGKVNGLIAKLRYAVP GVTLISPPPHHDIYSIEDLAQLIYDLKQVNPQALVSVKLVAEAGVGTIAAGVAKAYADLI TISGYDGGTGASPLTSIKYAGAPWELGLAETHQTLRGNDLRGKVRVQTDGGLKTGLDVIK AAILGAESFGFGTAPMIALGCKYLRICHLNNCATGVATQNDKLRKDHYIGTVDMVINFFT FVAEETREWLAKLGVRSLGELIGRTDLLEVLPGDTERQQYLDLSPLLGSSHIPADKPQFC EVDKNPPFDLGELAEKMVDMAMPAIRDQAGGEFSLDICNCDRSIGARVSGEIARLHGNQG MAAAPITFRFKGTAGQSFGVWNAGGLNLHLEGDANDYVGKGMTGGKVTIVPPAGSPFETQ HSAIVGNTCLYGATGGKLFAAGTAGERFAVRNSGAHAVVEGTGDHCCEYMTGGFVCVLGK TGYNFGSGMTGGFAYVLDMDNSFVDKLNHELVEIQRISGEAMEAYRSHLARVLAEYVDET GSEWGRELSENLDDYVRRFWLVKPKAANLKQLLSSTRANPQ