Protein Info for PP_5072 in Pseudomonas putida KT2440

Annotation: efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 transmembrane" amino acids 43 to 64 (22 residues), see Phobius details amino acids 77 to 97 (21 residues), see Phobius details amino acids 109 to 127 (19 residues), see Phobius details amino acids 134 to 155 (22 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details amino acids 248 to 269 (22 residues), see Phobius details amino acids 281 to 302 (22 residues), see Phobius details amino acids 312 to 329 (18 residues), see Phobius details amino acids 335 to 358 (24 residues), see Phobius details amino acids 370 to 391 (22 residues), see Phobius details amino acids 398 to 417 (20 residues), see Phobius details PF07690: MFS_1" amino acids 47 to 267 (221 residues), 121.7 bits, see alignment E=5e-39 amino acids 253 to 422 (170 residues), 54.1 bits, see alignment E=1.9e-18 PF00083: Sugar_tr" amino acids 76 to 223 (148 residues), 48.3 bits, see alignment E=1.1e-16

Best Hits

Swiss-Prot: 61% identical to YNFM_ECOLI: Inner membrane transport protein YnfM (ynfM) from Escherichia coli (strain K12)

KEGG orthology group: K08224, MFS transporter, YNFM family, putative membrane transport protein (inferred from 100% identity to ppu:PP_5072)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CV8 at UniProt or InterPro

Protein Sequence (425 amino acids)

>PP_5072 efflux transporter (Pseudomonas putida KT2440)
MLFSLRQRYPPVKTAVAPLLLEPKPAILTEMWIEKGTPAFMKTVLALFSGGFATFALLYC
VQPMMPLLSREFAINAAQSSLVLSVSTAMLAFGLLITGPISDRIGRKPVMVFALVCAALS
TLASAVMPSWELVLATRALVGLSLSGLAAVAMTYLSEEIHPQHIGLAMGLYIGGNAIGGM
SGRLITGVLIDFVSWHTAMLTIGGLALVAALVFWKVLPESRNFRAQTMNPRSLLDGFVMH
FKDAGLPWLFLEAFLLMGAFVTLFNYIGYRLLAEPYHMNQALVGLLSVVYLSGIYSSAQV
GALADKLGRRKVFWASIVVMAVGLLMTLASPLVMVIVGMLVFTFGFFGAHSVASSWIGRR
ALKAKGQASSLYLFCYYAGSSVAGTAGGVFWHQWGWNGIGLFIGSLLAVALMVALHLSKL
PPKTV