Protein Info for PP_5031 in Pseudomonas putida KT2440

Annotation: proline (histidine) APC transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 467 transmembrane" amino acids 16 to 37 (22 residues), see Phobius details amino acids 43 to 62 (20 residues), see Phobius details amino acids 88 to 118 (31 residues), see Phobius details amino acids 124 to 142 (19 residues), see Phobius details amino acids 154 to 176 (23 residues), see Phobius details amino acids 199 to 223 (25 residues), see Phobius details amino acids 243 to 263 (21 residues), see Phobius details amino acids 275 to 301 (27 residues), see Phobius details amino acids 332 to 353 (22 residues), see Phobius details amino acids 359 to 381 (23 residues), see Phobius details amino acids 402 to 423 (22 residues), see Phobius details amino acids 429 to 448 (20 residues), see Phobius details PF00324: AA_permease" amino acids 15 to 450 (436 residues), 379.3 bits, see alignment E=2.7e-117 PF13520: AA_permease_2" amino acids 18 to 439 (422 residues), 121 bits, see alignment E=6.6e-39

Best Hits

Swiss-Prot: 64% identical to PROY_SALTY: Proline-specific permease ProY (proY) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 100% identity to ppf:Pput_4905)

MetaCyc: 47% identical to phenylalanine:H+ symporter PheP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-56

Predicted SEED Role

"Histidine transport protein (permease)" in subsystem Histidine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88CZ8 at UniProt or InterPro

Protein Sequence (467 amino acids)

>PP_5031 proline (histidine) APC transporter (Pseudomonas putida KT2440)
MQQAQGLKRGLSARHIRFMALGSAIGTGLFYGSASAIQMAGPAVLLAYLIGGAAVFMVMR
ALGEMAVHNPVAGSFGHYATTYLGPMAGFILGWTYAFEMVIVAIADVTAFGIYMGFWFPE
VARWIWVLGIVFLIGGLNLCNVKVFGEMEFWLSLLKVGAIVAMILAGLGIMAFGFSQVGT
GHAVGMSNLFDHGGFMPNGVGGLIASFAVVMFAFGGIEIIGVTAGEAKDPQRVIPKAINA
VPLRILLFYVLTLFVLMCLYPWPQIGSQGSPFVQIFSNLGIGSAAAVLNVVVISAAISAI
NSDIFGAGRMMYGLAQQGHAPRGFSKLSKHGVPWMTVVVMGAALLIGVLLNYLIPENVFL
LIASIATFATVWVWLMILLTQVAMRRSMSREQVAQLKFPVPFWPYGPAMAIAFMVFIFGV
LGYFPDTQAALIVGVIWVVFLVASYLLWCKPRAGQGQPVAEPAELHR