Protein Info for PP_4967 in Pseudomonas putida KT2440

Annotation: methionine adenosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00438: S-AdoMet_synt_N
  • amino acids 6 to 101 (96 residues), 141.1 bits, E=2.2e-45
• TIGR01034: methionine adenosyltransferase
  • amino acids 6 to 393 (388 residues), 603 bits, E=1e-185
• PF02772: S-AdoMet_synt_M
  • amino acids 115 to 232 (118 residues), 155.9 bits, E=7.8e-50
• PF02773: S-AdoMet_synt_C
  • amino acids 234 to 365 (132 residues), 223.1 bits, E=1.7e-70

Best Hits

Swiss-Prot: 100% identical to METK_PSEPG: S-adenosylmethionine synthase (metK) from Pseudomonas putida (strain GB-1)

KEGG orthology group: K00789, S-adenosylmethionine synthetase [EC: 2.5.1.6] (inferred from 100% identity to ppg:PputGB1_5016)

MetaCyc: 74% identical to methionine adenosyltransferase (Escherichia coli K-12 substr. MG1655)
Methionine adenosyltransferase. [EC: 2.5.1.6]

Predicted SEED Role

"S-adenosylmethionine synthetase (EC 2.5.1.6)" in subsystem Methionine Biosynthesis or Methionine Degradation or Quorum Sensing: Autoinducer-2 Synthesis (EC 2.5.1.6)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• S-adenosyl-L-methionine biosynthesis (1/1 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (3/5 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• ethene biosynthesis I (plants) (1/3 steps found)
• 2'-deoxymugineic acid phytosiderophore biosynthesis (1/4 steps found)
• L-methionine salvage cycle III (5/11 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-methionine salvage cycle I (bacteria and plants) (5/12 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.6

Sequence Analysis Tools

See Q88D60 at UniProt or InterPro

Protein Sequence (396 amino acids)

>PP_4967 methionine adenosyltransferase (Pseudomonas putida KT2440)
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKYARVACETLVKTGVAIIAGEVTT
SAWVDLEDLVRKVIIDIGYNSSDVGFDGATCAVMNIIGKQSVDIAQGVDRSKPEDQGAGD
QGLMFGYASNETEVLMPAPICFSHRLVERQAEARKSGLLPWLRPDAKSQVTCRYENGKVV
GIDAVVLSTQHNPEVSQKDLQEAVMELIVKHTLPAELLHKGTQYHINPTGNFIIGGPVGD
CGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
SYAIGVAQPTSISINTFGTGKVSDDKIIQLVRECFDLRPYAITTMLDLLHPMYQETAAYG
HFGRTPQQKTVGDDTFTTFTWERTDRAQSLRDAAGL