Protein Info for PP_4953 in Pseudomonas putida KT2440

Annotation: putative oxidoreductase with FAD/NAD(P)-binding domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF01494: FAD_binding_3" amino acids 4 to 160 (157 residues), 23.2 bits, see alignment E=1.9e-08 PF00890: FAD_binding_2" amino acids 5 to 50 (46 residues), 26.2 bits, see alignment 2.4e-09 PF03486: HI0933_like" amino acids 5 to 391 (387 residues), 326.1 bits, see alignment E=7e-101 PF12831: FAD_oxidored" amino acids 5 to 138 (134 residues), 32.4 bits, see alignment E=3.4e-11 TIGR00275: flavoprotein, HI0933 family" amino acids 6 to 391 (386 residues), 423.9 bits, see alignment E=3e-131 PF13450: NAD_binding_8" amino acids 8 to 39 (32 residues), 28.4 bits, see alignment (E = 8e-10) PF22780: HI0933_like_1st" amino acids 188 to 338 (151 residues), 152 bits, see alignment E=7e-48

Best Hits

KEGG orthology group: K07007, (no description) (inferred from 100% identity to ppu:PP_4953)

Predicted SEED Role

"FIG00953568: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D74 at UniProt or InterPro

Protein Sequence (393 amino acids)

>PP_4953 putative oxidoreductase with FAD/NAD(P)-binding domain (Pseudomonas putida KT2440)
MHSTDVIILGAGAAGLMCAQLSARSGRRVLLLDHANKPGKKILMSGGGRCNFTNLYTEPA
NFLSHNAHFCKSALARYTQWDFIELVCKHGVAYHEKKLGQLFCDNKASDILDMLLAECDE
AGAEIRMHTSIEQIEKTESGYLLQTSGGQFACQSLVIATGGLSIPTLGATGFGYQVARQF
GHTLLPTRAGLVPFTITEPQLKAMCTELSGTSLDCTASCNGTSFRENLLFTHRGLSGPAI
LQISSFWEAGDTVEINLLPDRDALSWLQQMQAERANAELKTVLGEVFTRKLANLLAEQWF
ESKPMKQYTPAELAQIAEKLANWQVVPAGTEGYRTAEVTLGGVDTREVSSKTMESLKSPG
LYFIGEVLDVTGHLGGFNFQWAWASANAAAQFV