Protein Info for PP_4935 in Pseudomonas putida KT2440

Annotation: Lipid A export ATP-binding/permease protein MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 transmembrane" amino acids 23 to 48 (26 residues), see Phobius details amino acids 78 to 104 (27 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details amino acids 182 to 200 (19 residues), see Phobius details amino acids 265 to 288 (24 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 16 to 594 (579 residues), 879.6 bits, see alignment E=4.6e-269 PF00664: ABC_membrane" amino acids 32 to 307 (276 residues), 163.8 bits, see alignment E=7.1e-52 PF00005: ABC_tran" amino acids 372 to 522 (151 residues), 114.9 bits, see alignment E=4.5e-37

Best Hits

Swiss-Prot: 100% identical to MSBA_PSEPK: Lipid A export ATP-binding/permease protein MsbA (msbA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 100% identity to ppf:Pput_4807)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D92 at UniProt or InterPro

Protein Sequence (602 amino acids)

>PP_4935 Lipid A export ATP-binding/permease protein MsbA (Pseudomonas putida KT2440)
MAETPRPAEHTSSLKIYFRLLSYVKPYVGIFLLSIVGFVIFASTQPMLAGILKYFVDGLS
NPEAVLFPNVPYLRDLQLLQAVPLLIILIAAWQGLGSFLGNYFLAKVSLSLVHDLRVALF
NKLLVLPNRYFDNHNSGHLISRITFNVTMVTGAATDAIKVVIREGLTVVFLFAYLLWMNW
HLTLVMVAILPVIAVMVSIASKKFRKQSKKIQVAMGDVTHVASETIQGYRVVRSFGGEAY
EQQRFGQASQSNTDKQLRMTKTGSLYTPMLQLVIYSAMAALMFLVLFLRGDSTAGDLVAY
ITAAGLLPKPIRQLSEVSSTIQKGLAGAESIFEQLDEAPEVDTGTVEKERVEGRLEVRNL
SFTYPGTEREVLSDISFVAEPGQMIALVGRSGSGKSTLAALIPRFYHHDKGQILLDGVEI
EHYRLRNLRRHVSQVTQHVTLFNDTVANNIAYGDLAGAPRADIEAAAADAYAKEFVDRLP
KGFDTEVGENGVLLSGGQRQRLAIARALLKNAPLLILDEATSALDTESERHIQAALDHVM
QGRTTLVIAHRLSTIEKADQILVMDQGRLVERGTHTELLAANGHYARLHAMGLDEPAKAD
IT