Protein Info for PP_4928 in Pseudomonas putida KT2440

Annotation: 3-deoxy-D-manno-octulosonic acid transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 54% identical to KDTA_ECOL6: 3-deoxy-D-manno-octulosonic acid transferase (waaA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 100% identity to ppf:Pput_4800)

MetaCyc: 54% identical to KDO transferase (Escherichia coli K-12 substr. MG1655)
KDOTRANS2-RXN [EC: 2.4.99.13]; KDOTRANS-RXN [EC: 2.4.99.13, 2.4.99.12]

Predicted SEED Role

No annotation

MetaCyc Pathways

superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
Kdo transfer to lipid IV_A (Chlamydia) (2/5 steps found)
superpathway of Kdo₂-lipid A biosynthesis (16/25 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88D99 at UniProt or InterPro

Protein Sequence (423 amino acids)

>PP_4928 3-deoxy-D-manno-octulosonic acid transferase (Pseudomonas putida KT2440)
MNRTLYTLLFHLGLPLVALRLYLRARKAPAYGQRIGERFAFKLPAMRQGGIWVHAVSVGE
SIAAAPMVRALLKAYPELPITLTCMTPTGSERIRALFANEPRVQHCYLPYDLPWAAGRFL
DHVQPKLGIIMETELWPNHIHQCARRGIPVALANARLSERSARGYGRFARLTRPMLAEMS
LIAVQTETEAQRFRDLGARPERVQVTGSIKFDLTIDEQLLPRARALREQLGGGQRPVWIA
ASTHDGEDALILQAHRQLLQVHGDALLILVPRHPERFNAVHALCSEQFTTVRRSAGTLVD
AQTRVLLGDTMGELLFLYALADIAFVGGSLVPTGGHNPLEPAALALPVLMGPHVFNFLEI
SAMLREAGALQQVDDADGLAGAVRRLVELPQDAQRMGEAGRAVMRANQGALQRLLEGLGA
LIR