Protein Info for PP_4902 in Pseudomonas putida KT2440

Annotation: Oligoribonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to ORN_PSEPK: Oligoribonuclease (orn) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K13288, oligoribonuclease [EC: 3.1.-.-] (inferred from 99% identity to ppg:PputGB1_4954)

MetaCyc: 89% identical to oligoribonuclease (Pseudomonas aeruginosa)
RXN-24101

Predicted SEED Role

"3'-to-5' oligoribonuclease (orn)" in subsystem RNA processing and degradation, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DC5 at UniProt or InterPro

Protein Sequence (180 amino acids)

>PP_4902 Oligoribonuclease (Pseudomonas putida KT2440)
MQNPQNLIWIDLEMTGLDPDSDVIIEMATIVTDSELNTLAEGPVIAIHHSDEVLARMDEW
NTRTHGASGLTQRVRESKVSMAEAEAQTIAFLEQWVPKGKSPICGNSICQDRRFLYRHMR
NLENYFHYRNLDVSTLKELAARWAPDVRDSFKKGNTHLALDDIRESIAELRHYREHFIKL