Protein Info for PP_4892 in Pseudomonas putida KT2440

Annotation: integral membrane ATP-dependent zinc metallopeptidase / HflK-HflC complex

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 transmembrane" amino acids 80 to 102 (23 residues), see Phobius details PF12221: HflK_N" amino acids 13 to 64 (52 residues), 64.7 bits, see alignment 5.7e-22 TIGR01933: HflK protein" amino acids 100 to 361 (262 residues), 389.1 bits, see alignment E=4.7e-121 PF01145: Band_7" amino acids 102 to 273 (172 residues), 100.2 bits, see alignment E=1.5e-32

Best Hits

Swiss-Prot: 42% identical to HFLK_VIBPA: Protein HflK (hflK) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 100% identity to ppu:PP_4892)

MetaCyc: 44% identical to regulator of FtsH protease (Escherichia coli K-12 substr. MG1655)
3.4.24.-

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DD5 at UniProt or InterPro

Protein Sequence (405 amino acids)

>PP_4892 integral membrane ATP-dependent zinc metallopeptidase / HflK-HflC complex (Pseudomonas putida KT2440)
MGASLLYQMESAMAWNEPGGNSNNQDPWGGRRGGGGGGGDKKGPPDLDEAFRKLQDSLNG
MFGGSKKRGGGDRNVGKGGGLGLLGIGLAVLAAIWLYSAVYVVDEQEQAVVLRFGKYYET
VGPGLNIYFPPIDRKYMENVTRERAYTKQGQMLTEDENIVEVPLTVQYKISNLQDFVLNV
DQPEVSLQHATDSALRHVVGSTSMDQVLTEGREQMAVDIRERLQRFLDNYRTGITVTQVN
VQSAAAPREVQEAFDDVIRAREDEQRARNQAESYANGVVPEARGQAQRIIEDANGYRDEV
IARAKGEADRFTKLLAEYRKAPDVTRERLYLETMQEVYSNSSKVMVATKDGQSNLLYLPL
DKMVEGSRKPSVPTTSASPSANDAAARAAQDMQQQQQPLRTRESR