Protein Info for PP_4891 in Pseudomonas putida KT2440

Annotation: integral membrane ATP-dependent zinc metallopeptidase / HflK-HflC complex

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF01145: Band_7" amino acids 25 to 200 (176 residues), 121.5 bits, see alignment E=2.2e-39 TIGR01932: HflC protein" amino acids 154 to 284 (131 residues), 155.1 bits, see alignment E=9.6e-50

Best Hits

KEGG orthology group: K04087, membrane protease subunit HflC [EC: 3.4.-.-] (inferred from 100% identity to ppu:PP_4891)

Predicted SEED Role

"HflC protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DD6 at UniProt or InterPro

Protein Sequence (289 amino acids)

>PP_4891 integral membrane ATP-dependent zinc metallopeptidase / HflK-HflC complex (Pseudomonas putida KT2440)
MSNRSLIALIAAVVLAIVAWNSFYIVSQTERAVLLRFGKVVQADVQPGLHVKIPYVNQVR
RFDARLMTLDAPTQRFLTLEKKAVMVDAYAKWRVKDAERFYTATSGMKQIADERLSRRLE
SGLRDQFGKRTLHEVVSGERDALMADITASLNRMASKELGIEVVDVRVKAIDLPKEVNRS
VFDRMSTEREREAREHRAKGNELAEGIRADADRQRRVLLAEAYREAEETRGDGDAQAAAI
YAKAYSQDADFYAFHRSLQAYRESFSSKSDVMVLDPKNEFFRYLDKSRP