Protein Info for PP_4889 in Pseudomonas putida KT2440
Annotation: Adenylosuccinate synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PURA_PSEPG: Adenylosuccinate synthetase (purA) from Pseudomonas putida (strain GB-1)
KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 100% identity to ppu:PP_4889)MetaCyc: 71% identical to adenylosuccinate synthetase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate synthase. [EC: 6.3.4.4]
Predicted SEED Role
"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
- superpathway of purine nucleotide salvage (13/14 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
- adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Purine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.4.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88DD8 at UniProt or InterPro
Protein Sequence (430 amino acids)
>PP_4889 Adenylosuccinate synthetase (Pseudomonas putida KT2440) MGKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVINGEKTVLHLIPSGI LREGVQCLIGNGVVVAPDALMREITKLEEKGVPVRERLRISPAAPLILSYHVALDQAREK ARGEAKIGTTGRGIGPAYEDKVARRGLRVGDLFHRERFAAKLGELLDYHNFQLVNYYKEP AIDFQQTLDECMAYAEQLKPMMLDVTAELHNLRRAGKDIMFEGAQGSLLDIDHGTYPYVT SSNTTAGGISTGSGVGPMYLDYILGITKAYTTRVGSGPFPTELFDETGATLAKRGHEFGS TTGRARRCGWFDAVILRRAIDVNSISGICLTKLDVLDGLETINICVGYKNENGAVIDAPS DADSYIGLEPVYEEMPGWSESTLGVKTLEELPQAARDYIKRIEELVGAPIDIISTGPDRN ETIVLRHPFA