Protein Info for PP_4872 in Pseudomonas putida KT2440

Annotation: putative enzyme of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 784 PF08497: Radical_SAM_N" amino acids 41 to 396 (356 residues), 459 bits, see alignment E=1.1e-141 TIGR03904: uncharacterized radical SAM protein YgiQ" amino acids 41 to 661 (621 residues), 870.6 bits, see alignment E=2.2e-266 PF04055: Radical_SAM" amino acids 397 to 602 (206 residues), 51.9 bits, see alignment E=1.5e-17 PF11842: DUF3362" amino acids 611 to 770 (160 residues), 162.8 bits, see alignment E=1e-51

Best Hits

Swiss-Prot: 100% identical to Y4872_PSEPK: UPF0313 protein PP_4872 (PP_4872) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4872)

Predicted SEED Role

"Fe-S OXIDOREDUCTASE (1.8.-.-)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DF3 at UniProt or InterPro

Protein Sequence (784 amino acids)

>PP_4872 putative enzyme of unknown function (Pseudomonas putida KT2440)
MCYIPRPRFPCTPEPVTDMQAAKPLYDYPKYWAECFGPAPFLPMSREEMDLLGWDSCDII
IVTGDAYVDHPSFGMAIIGRLLEAQGFRVGIIAQPNWQSKDDFMKLGEPNLFFGVAAGNM
DSMINRYTADKKIRSDDAYTPGGLAGSRPDRASLVYSQRCKEAYKHVPIVLGGIEASLRR
IAHYDYWQDKVRHSILIDASADILLFGNAERAVVEVAQRLSNGEKIETITDVRGTAFVRR
DTPQGWYEIDSTRIDRPGRVDKIINPYVNTQDTQACAIEQAKGDQEDPNEAKVVQILDSP
AVTREKSVIRLPSFEKVRNDPVLYAHANRVLHLETNPGNARALVQKHGEVDVWFNPPPIP
MSTEEMDYVFGMPYARVPHPAYGKERIPAYEMIRFSVNIMRGCFGGCTFCSITEHEGRII
QNRSHESILHEIEEMRDKVPGFTGVVSDLGGPTANMYRIACKSPEIEKYCRKPSCVFPGI
CENLNTDHSSLIELYRKARALPGVKKILIASGLRYDLAVESPEYVKELVTHHVGGYLKIA
PEHTERGPLDKMMKPGIGTYDRFKRMFEKFSKEAGKEQYLIPYFIAAHPGTTDEDMMNLA
LWLKGNGFRADQVQAFYPSPMASATAMYHSGKNPLRKVTYKSEGVEIVKSDEQRRLHKAF
LRYHDPKGWPMLREALQRMGRADLIGPGKHQLIPLHQPQTDTYQSARRKNSTPAGSHKVG
KDQKILTQHTGLPPRGSDGSKPWDKREKAKAEAFARNQQAAKERKEASKGGKGNKKPRQP
VIPR