Protein Info for PP_4854 in Pseudomonas putida KT2440
Annotation: Lipase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01046, triacylglycerol lipase [EC: 3.1.1.3] (inferred from 100% identity to ppu:PP_4854)Predicted SEED Role
"Lipase precursor (EC 3.1.1.3)" in subsystem Cytolysin and Lipase operon in Vibrio (EC 3.1.1.3)
MetaCyc Pathways
- triacylglycerol degradation (2/3 steps found)
- retinol biosynthesis (1/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.3
Use Curated BLAST to search for 3.1.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88DH1 at UniProt or InterPro
Protein Sequence (315 amino acids)
>PP_4854 Lipase (Pseudomonas putida KT2440) MVMMAAVVLARRTRKGAAAMQQDLATRYPLVLVPGMLGFVRMVLYPYWFGIVPALRKGGA QVFPVQVSPLHSSEVRGEQLLAIIDDICQRTGVDKVNLIGHSQGALSARYAAAKRPGRVA SVTSVAGPNHGSELADHLERTAPGNSPQGRILKAVLHGFAVLLVWLETGWRRDPLPVDVH ASHQSLTSAGVALFNQAYPQGLPQTWGGEGAYEVNGVRYYSWSGTLQPGLTDQGRNRFDG SSRFCRLFARSFIKEKGHCDGMVGRFSSHLGQVIGDDYPLDHLDIVNQSLGAVGKGAEPV RLFTEHAARLKAAGL