Protein Info for PP_4822 in Pseudomonas putida KT2440

Annotation: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 11 to 535 (525 residues), 767.8 bits, E=2.9e-235
• PF02142: MGS
  • amino acids 22 to 135 (114 residues), 87.3 bits, E=6.3e-29
• PF01808: AICARFT_IMPCHas
  • amino acids 140 to 466 (327 residues), 408.6 bits, E=2e-126

Best Hits

Swiss-Prot: 100% identical to PUR9_PSEP1: Bifunctional purine biosynthesis protein PurH (purH) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 100% identity to ppu:PP_4822)

MetaCyc: 68% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See Q88DK3 at UniProt or InterPro

Protein Sequence (535 amino acids)

>PP_4822 phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (Pseudomonas putida KT2440)
MTDQTTRLPVRRALISVSDKTGILEFARELQQLGVEILSTGGTYKLLKDNGVNAVEVADY
TGFAEMMDGRVKTLHPKIHGGILGRRGTDDAIMNEHGIKPIDLVAVNLYPFEATISKPGC
DLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDYAGIVEGLKAGGLTYAQRFDLMLKA
FEHTAAYDGMIANYMGTIDQAKDTLSTEARSEFPRTFNSQFVKAQEMRYGENPHQSAAFY
VEAKKGEASISTAVQLQGKELSFNNVADTDAALECVKSFVKPACVIVKHANPCGVAVVPE
DEGGIRKAYDLAYATDTESAFGGIIAFNRELDGETAKAIVDRQFVEVIIAPKISQAARDV
VAAKQNVRLLECGEWPAERAAGWDFKRVNGGLLVQSRDIGMITADDLKIVTKRAPTEQEI
HDLVFAWKVAKFVKSNAIVYAKQRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVQGAVMA
SDAFFPFRDGIDNAAKVGISAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH