Protein Info for PP_4815 in Pseudomonas putida KT2440

Annotation: putative Membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 238 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 40 to 63 (24 residues), see Phobius details amino acids 72 to 92 (21 residues), see Phobius details amino acids 99 to 124 (26 residues), see Phobius details amino acids 151 to 177 (27 residues), see Phobius details amino acids 189 to 205 (17 residues), see Phobius details amino acids 212 to 236 (25 residues), see Phobius details PF04172: LrgB" amino acids 23 to 235 (213 residues), 251.3 bits, see alignment E=3.4e-79

Best Hits

Swiss-Prot: 30% identical to Y1298_HAEIN: Uncharacterized protein HI_1298 (HI_1298) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 99% identity to ppg:PputGB1_4868)

Predicted SEED Role

"LrgA-associated membrane protein LrgB" in subsystem Murein hydrolase regulation and cell death

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DL0 at UniProt or InterPro

Protein Sequence (238 amino acids)

>PP_4815 putative Membrane protein (Pseudomonas putida KT2440)
MMLDWQGALDAVIHHPLFGIGITLGAYQVVLAAYEKTRWIFLQPVLVSMLLVIGVLLLCG
IDYSEYRKSTEIMNILLGPATVALAVPLYLNLRRIRQLFWPTFTTLVVGGLFATLCCLVL
GWWFGAEHMILMTMAPKSVTSPIAMLVAEQIGGVAALAAVFVLITGVIGAIFGPALLSRF
GVHSPEARGMSLGVTAHAVGTSVALQESDECGAFAALAMSLMGVATAVFLPLAVSLVA