Protein Info for PP_4780 in Pseudomonas putida KT2440

Annotation: putative Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 PF18158: AidB_N" amino acids 14 to 168 (155 residues), 204.8 bits, see alignment E=2e-64 PF02770: Acyl-CoA_dh_M" amino acids 182 to 279 (98 residues), 53.2 bits, see alignment E=6.9e-18 PF00441: Acyl-CoA_dh_1" amino acids 290 to 444 (155 residues), 91.5 bits, see alignment E=1.6e-29 PF08028: Acyl-CoA_dh_2" amino acids 316 to 426 (111 residues), 33.9 bits, see alignment E=9.1e-12 PF22924: ACOX_C_alpha1" amino acids 373 to 444 (72 residues), 33.7 bits, see alignment E=8.1e-12

Best Hits

KEGG orthology group: K09456, putative acyl-CoA dehydrogenase (inferred from 100% identity to ppu:PP_4780)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DP4 at UniProt or InterPro

Protein Sequence (550 amino acids)

>PP_4780 putative Acyl-CoA dehydrogenase (Pseudomonas putida KT2440)
MSLHQYAETHEVTNQPPPLDGANLYRLDLPLQEWSRRFGAGWAESRIDAYGALAGGPLMQ
AGFLANAHKPEFSSHDRYGHRIDLVEFHPAYHELMRTAVEHGLPSLPWAEPRAGAHVARA
SMTYLHSQAEAGTGCPLTMTFAAVPALRLQPELAEYWLPKILACEYDPRNVGDRHKAGVT
LGMAMTEKQGGTDVRANTTRAYPVGAPGPGQAYELVGHKWFCSAPMCDAFLTLAQTEKGL
SCFLLPRHRPDDNRNQFYIQRLKNKLGNSSNASSEVEFRGALAWMIGEEGRGVPTIIEMV
AMTRFDCMVGSSALMRQALTQAAHHCAHRKVGGRVLNEQPLMQNVLADLALESEAALALS
LRMGQALEQLDDPQQAHFARLVTAVGKYWICKRAPAMINEAAECLGGAGYVEDSILPRLY
REAPVNSTWEGSGNVQCLDVLRALSKEPGVLDALFVELGDGHGDPRLAAHIGNLKGAFAD
TGDMQYRARQLTEDIALALQAKLLLEAGNADVSDAFIGSRLAGGGRVYGALPRGVNVMAL
VTRATPAWPL