Protein Info for PP_4774 in Pseudomonas putida KT2440

Annotation: divalent cation efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 290 transmembrane" amino acids 18 to 36 (19 residues), see Phobius details amino acids 42 to 66 (25 residues), see Phobius details amino acids 83 to 105 (23 residues), see Phobius details amino acids 117 to 139 (23 residues), see Phobius details amino acids 161 to 178 (18 residues), see Phobius details amino acids 184 to 201 (18 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 16 to 289 (274 residues), 216.7 bits, see alignment E=2e-68 PF01545: Cation_efflux" amino acids 18 to 209 (192 residues), 138.8 bits, see alignment E=2.1e-44 PF16916: ZT_dimer" amino acids 216 to 290 (75 residues), 80.3 bits, see alignment E=9.5e-27

Best Hits

Swiss-Prot: 49% identical to FIEF_ESCF3: Ferrous-iron efflux pump FieF (fieF) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4774)

MetaCyc: 48% identical to Zn2+/Fe2+/Cd2+ exporter (Escherichia coli K-12 substr. MG1655)
RXN0-6; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DP9 at UniProt or InterPro

Protein Sequence (290 amino acids)

>PP_4774 divalent cation efflux transporter (Pseudomonas putida KT2440)
MITTPAEHQRLLRLATRASLAVAGILVLSKALAWWLSGSVSLLAGLTDSALDAVASFLNL
LAVHYALRPADDDHRFGHGKAEALAGMAQALFIGGSAVLIGVQAVERLYTPQPLGDTSIG
IAVMLLSLVLTVALLALQYKVIRLTGSTAVRADSLHYRSDLLLNGSILLALLLTRFGWPQ
LDALFGLGIAFYILWSALQIARESTAILMDKALPGDVGEDMTALVLAVAGVKGVHDLRTR
VSGNQWFVQLHLELPGQLPLHEAHSLCVEASRVIRQRYPQADVMVHADPV