Protein Info for PP_4772 in Pseudomonas putida KT2440

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 842 TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 841 (838 residues), 1310.8 bits, see alignment E=0 PF00270: DEAD" amino acids 13 to 166 (154 residues), 39.9 bits, see alignment E=7.5e-14 PF00271: Helicase_C" amino acids 209 to 333 (125 residues), 48.6 bits, see alignment E=1.8e-16 PF24473: CON_HrpB" amino acids 595 to 629 (35 residues), 44.9 bits, see alignment (E = 1.6e-15) PF08482: HrpB_C" amino acids 697 to 828 (132 residues), 182.5 bits, see alignment E=9e-58

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 100% identity to ppu:PP_4772)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DQ1 at UniProt or InterPro

Protein Sequence (842 amino acids)

>PP_4772 ATP-dependent helicase HrpB (Pseudomonas putida KT2440)
MISLPIDAALPALRQALENRDEVVLEAPPGAGKTTRVPLALLHEPWLAGQSILMLEPRRL
AARAAAERLASELGEQVGETVGYRIRLDSKVGPKTRIEVVTEGILTRRLQADPALEGVGL
LIFDEFHERSLDADLALALSLNGRELLRDDPPLKILLMSATLEGERLSRLLDDAPVVSSE
GRMHPVDIRWGRPFQPGEFIEPRVVDCVLHALADQAGSVLVFLPGQAEIRRVHQSLQEVL
GARPEILLCPLHGELDLNAQRAAIDPPAKGLRKVVLATNIAETSLTIDGVRVVIDAGLAR
VPRFDPGSGMTRLDTQRISRASATQRAGRAGRLEPGVCYRLWSEAQHDQLAAHGSAEILQ
ADLAGLALQLARWGVTPEQLRWLDQPPAAAYAQAQDLLARLSAFRPGSRDTLSEHGQAMA
ELPAHPRIAHLLLRGQDLGLAQMACDVAALLGERDIQRGGGADLHSRLALVSGESKAARG
GQGGVQRARQLARQYRGLLRGKVGAPVADPDHPRWLGALLALAYPDRVAQQRRAGGAEYR
LANGRAALFGEVDALMKCPWLVIADLGSRQGQREERIYLAAEFDPALLEGVLAEQVERVD
ILDWDEREQVLRAERQVKVGELVLSREPLPGLDDEARTRALLGLVRRKGLNLLTWTPELR
QWQARVALLRQLDLQKDGHSEWPDLGDEALLASLEDWLQPYLSKVSRLSHFAALDLPSML
RNLLPWPLPQRLEEQAPAHLAVPSGSNIRLDYSETPPILAVRLQELFGLADTPRIANGRQ
QVKLHLLSPARRPVQVTQDLASFWRTTYAEVKKDLKGRYPKHYWPDDPLVAEATARAKPR
GS