Protein Info for PP_4756 in Pseudomonas putida KT2440

Annotation: gamma-aminobutyrate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 48 to 65 (18 residues), see Phobius details amino acids 96 to 121 (26 residues), see Phobius details amino acids 127 to 146 (20 residues), see Phobius details amino acids 153 to 175 (23 residues), see Phobius details amino acids 200 to 222 (23 residues), see Phobius details amino acids 242 to 263 (22 residues), see Phobius details amino acids 286 to 309 (24 residues), see Phobius details amino acids 337 to 356 (20 residues), see Phobius details amino acids 362 to 382 (21 residues), see Phobius details amino acids 402 to 424 (23 residues), see Phobius details amino acids 434 to 453 (20 residues), see Phobius details PF00324: AA_permease" amino acids 18 to 429 (412 residues), 394.7 bits, see alignment E=5.8e-122 PF13520: AA_permease_2" amino acids 19 to 441 (423 residues), 142.3 bits, see alignment E=2.2e-45

Best Hits

Swiss-Prot: 67% identical to BAUD_PSEAE: Probable GABA permease (bauD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 99% identity to ppg:PputGB1_4765)

MetaCyc: 59% identical to 4-aminobutanoate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-384; TRANS-RXN-57

Predicted SEED Role

"Gamma-aminobutyrate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DR7 at UniProt or InterPro

Protein Sequence (463 amino acids)

>PP_4756 gamma-aminobutyrate permease (Pseudomonas putida KT2440)
MNTVGSDGNLAQGFKPRHVTMLSIAGIIGAGLFVGSGHAIAAAGPATIISYFVAGTLVVL
VMRMLGEMAVAHPDTGSFSTYADQAIGRWAGYTIGWLYWWFWVLVIPIEALAAGHVLNAW
FPQVDSWIFALASVLLLAGTNLFSVAKYGEFEFWFAILKVTAILGFIGLGFAALMDWLPN
REVSGLSGLMAEYGGFAPKGWSAVVGAFITVMFSFIGTEAVTIAASESSDPSRNIAKATR
SVIWRISTFYILSIFVIISVVPWNDPQLAVVGSYQRALEIMNIPNAAFMVDLVVLVAVTS
CMNSSIYIASRMMFSLAKRGDAPAFLNKTSKVGVPRAAVFGSTLIGAAIAVLNYFAPKGV
FEFLLASSGAIALLVYMVIAISQLRMRRRLERENTELKFRMWLFPYLTWAVIIFIAGALA
VMMYTPEHRAEVSSTLGLAIVISFLGIVTSRGHAQPVAARSMG