Protein Info for PP_4725 in Pseudomonas putida KT2440

Annotation: 4-hydroxy-tetrahydrodipicolinate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00036: 4-hydroxy-tetrahydrodipicolinate reductase
  • amino acids 3 to 265 (263 residues), 341.5 bits, E=2e-106
• PF01113: DapB_N
  • amino acids 3 to 125 (123 residues), 150.9 bits, E=1.9e-48
• PF05173: DapB_C
  • amino acids 128 to 264 (137 residues), 170.3 bits, E=2e-54

Best Hits

Swiss-Prot: 100% identical to DAPB_PSEPK: 4-hydroxy-tetrahydrodipicolinate reductase (dapB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00215, dihydrodipicolinate reductase [EC: 1.3.1.26] (inferred from 99% identity to ppg:PputGB1_4726)

MetaCyc: 66% identical to 4-hydroxy-tetrahydrodipicolinate reductase (Escherichia coli K-12 substr. MG1655)
RXN-14014 [EC: 1.17.1.8]

Predicted SEED Role

"4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8)" (EC 1.17.1.8)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.1.8 or 1.3.1.26

Sequence Analysis Tools

See Q88DU4 at UniProt or InterPro

Protein Sequence (267 amino acids)

>PP_4725 4-hydroxy-tetrahydrodipicolinate reductase (Pseudomonas putida KT2440)
MRRIAVMGAAGRMGKTLIEAVQQTPGAGLTAAIDRPDSSLVGADAGELAALGRIGVLLSD
DLAKVADEFDVLIDFTHPSVTLKNLAFCRKHGKAMIIGTTGFTVEEKQLLAEAGKDIPIV
FAANFSVGVNLSLKLLDMAARVLGDDVDIEIIEAHHRHKVDAPSGTALRMGEVVANALGR
DLQEVAVYGREGQTGARDRKTIGFATVRAGDVVGDHTVLFAAEGERLEITHKASSRMTFA
KGAVRAALWLDGREPGLYDMQDVLELR