Protein Info for PP_4694 in Pseudomonas putida KT2440

Annotation: Glutamyl-Q tRNA(Asp) synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details TIGR03838: glutamyl-queuosine tRNA(Asp) synthetase" amino acids 11 to 275 (265 residues), 370.7 bits, see alignment E=2.2e-115 PF00749: tRNA-synt_1c" amino acids 14 to 236 (223 residues), 151 bits, see alignment E=1.9e-48

Best Hits

Swiss-Prot: 100% identical to GLUQ_PSEPK: Glutamyl-Q tRNA(Asp) synthetase (gluQ) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01894, glutamyl-Q tRNA(Asp) synthetase [EC: 6.1.1.-] (inferred from 99% identity to ppf:Pput_4558)

MetaCyc: 54% identical to glutamyl-Q tRNAAsp synthetase (Escherichia coli K-12 substr. MG1655)
2.4.1.M62 [EC: 2.4.1.M62]

Predicted SEED Role

"glutamyl-Q-tRNA synthetase" in subsystem Queuosine-Archaeosine Biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.-

Use Curated BLAST to search for 2.4.1.M62 or 6.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DX4 at UniProt or InterPro

Protein Sequence (300 amino acids)

>PP_4694 Glutamyl-Q tRNA(Asp) synthetase (Pseudomonas putida KT2440)
MPSLTMTDSSYIGRFAPTPSGFLHFGSLVAALASWLDARAVNGRWLLRMEDTDPPREMPG
ARDAILQTLERYGLHWDGEVVFQSQRHDAYAAVVDRLFNMGLAYACTCSRKQLESYNGIY
PGFCRNAGHAREGAAIRLRVPELIYRFTDRVQGEYQQHLGREVGDFVIQRRDGLYAYQLA
VVLDDAWQGVTDIVRGADLLDNTPRQLYLQELLGFSQPRYLHIPLIVQPDGHKLGKSYRS
PPLQAEHATPLLLRALRALGQETDPELLLATPAEVLAVARAQWRPEAIAQRTTVPEADLR