Protein Info for PP_4667 in Pseudomonas putida KT2440

Annotation: methylmalonate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 15 to 491 (477 residues), 764.7 bits, E=1.8e-234
• PF00171: Aldedh
  • amino acids 23 to 487 (465 residues), 484.5 bits, E=1.5e-149

Best Hits

Swiss-Prot: 60% identical to MMSA_BOVIN: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6A1) from Bos taurus

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 100% identity to ppf:Pput_4531)

MetaCyc: 72% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• β-alanine degradation II (2/2 steps found)
• L-valine degradation I (6/8 steps found)
• β-alanine degradation I (1/2 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• 2,4-dinitrotoluene degradation (1/7 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Sequence Analysis Tools

See Q88E01 at UniProt or InterPro

Protein Sequence (508 amino acids)

>PP_4667 methylmalonate-semialdehyde dehydrogenase (Pseudomonas putida KT2440)
MNAPQSPNSTKVEQVKLLIDGQWVESKTTEWRDIVNPATQEVLARVPFATVEEVDAAVAA
AQRAFQTWRNTPIGARMRIMLKLQALIREHTKRIAQVLSAEQGKTLADAEGDIFRGLEVV
EHAASIGTLQMGEFAENVAGGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIVCGNT
FVLKPSEQDPLSTMLLVELALEAGVPAGVLNVVHGGKQVVDAICTHQDIKAISFVGSTEV
GTHVYNLGSQHGKRVQSMMGAKNHAVVLPDANRTQTINALVGAAFGAAGQRCMATSVAVL
VGKAREWLPDIKEAASKLKVNAGCEPGTDVGPVVSKRAKERVLGLIESGIKEGAKLELDG
RDVKVPGYEQGNFVGPTLFSGVKTDMQVYTQEIFGPVLVTLEVDTLDEAIALVNANPFGN
GTGLFTQSGAAARKFQSEIDIGQVGINIPIPVPVPFFSFTGSRGSKLGDLGPYGKQVVQF
YTQTKTVTARWFDDDSVNDGVNTTISLR