Protein Info for PP_4659 in Pseudomonas putida KT2440

Annotation: gamma-glutamyltranspeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 31 to 546 (516 residues), 517.3 bits, see alignment E=2e-159 PF01019: G_glu_transpept" amino acids 44 to 549 (506 residues), 580.6 bits, see alignment E=1.6e-178

Best Hits

Swiss-Prot: 80% identical to GGT_PSEAE: Glutathione hydrolase proenzyme (ggt) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 100% identity to ppu:PP_4659)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88E09 at UniProt or InterPro

Protein Sequence (555 amino acids)

>PP_4659 gamma-glutamyltranspeptidase (Pseudomonas putida KT2440)
MRIVPFQYLALAAAILSCSSVYAATLEGGAVAAPDQYGAQVAADILKKGGNAVDAAVATA
FTLAVTYPEAGNIGGGGFMTLFVDGKPYFLDYREVAPKAATRNMYLDDKGEVIENLSLVG
ARAAGVPGTVMGLWEAHQKFGKLPWSELLTPAIGYAKNGFKVAEKQYQYRNDAQGMFKTA
TNFNDYFGNMKVGELFKQPEMAQTLERIADKGVSEFYQGKTADLLVAQMQADKGLITKED
LKDYKAVWREPMAVSWRGNVVYTAPPPSSGGVALAQLLGIKEDRAADFKGVAHNSAQYIH
LLAEIEKRVFADRADYLGDPAFTKVPVDQLVAKDYLAKRAAQVNPKAISDTDKVKPGLEP
HQTTHFSIVDKQGNAVSNTYTLNLDYGSGVVVKGAGFLLNDEMDDFSAKPGAANAFGVVG
GDANAIEPGKRMLSSMSPSLMTRDGKVELVIGTPGGSRIFTSIFQVMNNLYDYGMPLDKA
VAAQRVHHQLLPKDTIYFDSYAPLTGPVADELKKMGYVLEDQGWEMGDIQAIRVDGAKLE
TASDPRGRGVGMIVK