Protein Info for PP_4596 in Pseudomonas putida KT2440
Annotation: putative oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to ppu:PP_4596)Predicted SEED Role
"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase
MetaCyc Pathways
- formate oxidation to CO2 (1/1 steps found)
- purine nucleobases degradation II (anaerobic) (16/24 steps found)
- oxalate degradation VI (1/4 steps found)
- oxalate degradation III (1/5 steps found)
- superpathway of C1 compounds oxidation to CO2 (5/12 steps found)
- purine nucleobases degradation I (anaerobic) (6/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.2
Use Curated BLAST to search for 1.2.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88E70 at UniProt or InterPro
Protein Sequence (776 amino acids)
>PP_4596 putative oxidoreductase (Pseudomonas putida KT2440) MSQDEHIRDYKGAAAGWGALKSVTKSWLGSDNAFKNLRAMLKTNQNGGFDCPGCAWGESP ESDMVKFCENGAKAVNWEATGRSVDPAFFAKYSVSALKEQTDYWLEYQGRLTHPMRYDAA TDHYVETTWQEAFELVARHLRALQSPDEAEFYTSGRASNEAAFLYQLFVRAYGTNNFPDC SNMCHEASGAGMSETLGVGKGTVVFHDLELADAIFVIGQNPGTNHPRMLEPLREAVKRGA QVVCFNPLKERGLERFQHPQHPFEMLSNGSEPTSSAYFRPALGGDMAAMRGIAKYLLQWE REAQAKGEPAVFDHAFIAEHTSGVDDYLAAVDATSWEHIVKQSGLTLAEIELAARMYRKA ERVIMCWAMGVTQHRHSVPTVQEIVNLQLLRGNVGKPGAGLSPVRGHSNVQGDRTMGIDE KPSAALLDAIEQRFQFSVPRTHGHNAVLAIKAMEEGRAKVFIGLGGNFAQATPDTARTHA ALQNCALTVQISTKLNRSHLITGRDALILPCLGRTEIDLQAEGPQGVTVEDTFSMVHISN GQLRPRSPHMRSEPWIIAGMAKATLGNQPIDWEYAVADYNRIRDMIADVIPGFTGFNERL NSPGGFHLGNNAADRNFRTATGKARFMPHALPEELVNAKVLARGDKPDLILQTLRSHDQY NTTLYGLDDRYRGVFGLREVVFVNEADIRRLGFEPGEQVDLVSLWEDGVERRVSGFRLVA YDVPEGQAAAYYPETNPLVPLESYGEGTYTPTSKFVAIKVEKAKAGNRIAAVLASD