Protein Info for PP_4592 in Pseudomonas putida KT2440

Annotation: putative metal dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 545 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details amino acids 42 to 66 (25 residues), see Phobius details amino acids 73 to 93 (21 residues), see Phobius details amino acids 119 to 137 (19 residues), see Phobius details amino acids 148 to 170 (23 residues), see Phobius details PF08019: EptA_B_N" amino acids 53 to 202 (150 residues), 177.5 bits, see alignment E=1.7e-56 PF00884: Sulfatase" amino acids 233 to 521 (289 residues), 236.5 bits, see alignment E=4.4e-74

Best Hits

KEGG orthology group: K03760, phosphoethanolamine transferase (inferred from 100% identity to ppu:PP_4592)

Predicted SEED Role

"membrane protein, putative"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88E74 at UniProt or InterPro

Protein Sequence (545 amino acids)

>PP_4592 putative metal dependent hydrolase (Pseudomonas putida KT2440)
MLNFKSLRTEWVTLLASLYLLIGFNMFLWEHLQAVVPAGLSGLWLSLAFAVLMLFAFNLI
LTLIAFRYVVKPVLIVLFMSGAGVAYFMNQYGVLIDAGMFRNMAETNIAEVRDLMSFKFA
AYILLLGVLPSVLLWKAQIAYRPWHRELLGKLVVSGACVVTIGSVALVNYQGLSSLFRNH
HELRLMLTPSNIVGASIGYVDERVGTASRPFQHYGEDAKRDAAWLKHERKSLTVLVVGES
ARADHFGVLGYDRDTTPNLAREQGLLAFSDVHSCGTETAVSVPCMFSGMKRKDYDARVAK
NREGLLDILQRAGLAVQWRDNQSGCKGTCDRVQFIDVSNLKDPQLCADGECHDQILLQGL
DALIDNLDKDTVLVLHQMGSHGPEYFKRYPSDGERFTPVCQSNALNQCSEEEIINGYDNT
LAYTDKVLASLIDTLRSKQDKVDTAMIYLSDHGESLGEYNLFLHGTPYAIAPEQQKHIPL
LTWFSDSYKEDFGVDTDCLARLSDAPLSQDNLFHSMLGLLQVHTELYQQSLDMFAGCRPW
LAAKR