Protein Info for PP_4553 in Pseudomonas putida KT2440

Annotation: RNA polymerase sigma-70 factor, ECF subfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 8 to 161 (154 residues), 44.6 bits, see alignment E=5.8e-16 PF04542: Sigma70_r2" amino acids 9 to 75 (67 residues), 36.1 bits, see alignment E=6.8e-13 PF08281: Sigma70_r4_2" amino acids 108 to 159 (52 residues), 34.2 bits, see alignment 2.4e-12 PF20239: DUF6596" amino acids 177 to 276 (100 residues), 127.2 bits, see alignment E=4.1e-41

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 100% identity to ppu:PP_4553)

Predicted SEED Role

"RNA polymerase sigma-70 factor, ECF subfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EB3 at UniProt or InterPro

Protein Sequence (406 amino acids)

>PP_4553 RNA polymerase sigma-70 factor, ECF subfamily (Pseudomonas putida KT2440)
MRAEVEAVYRQESRRILATLIRLLGDFDLAEEAMHDAFFIAVERWQRDGIPANPRAWLVS
VGRFKAIDALRRRVRFDRSQADLSMLLEGQAQDPSEEELLADDRLRLIFTCCHPALSADA
QVPLTLREVCDLTTEQIARAFLQSPATIAQRIVRAKAKIRDAGIPYQVPELSELPERLES
VLRVIYLVFNEGYSASSGETLLQQALSDEAIRLARLLVQLLPDPEAVGLLALMLLQASRQ
RARIDGQGDLVVLEQQDRSLWDRQFIAEGCALVRQALQSRAFGAYTVQAAIAAVHAEAAT
AEETDWGEIVGLYDVLQRYWPSAVVALNRAVALAKRDGPEAGLREVEGILARGELLDYHL
AHAARAELHCQLGQVQEARAAWQQALALTRQAPERRHIERRLQAIE