Protein Info for PP_4551 in Pseudomonas putida KT2440

Annotation: Hydrolase, alpha/beta fold family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 PF12146: Hydrolase_4" amino acids 26 to 289 (264 residues), 200.2 bits, see alignment E=6.4e-63 PF00561: Abhydrolase_1" amino acids 30 to 284 (255 residues), 58.2 bits, see alignment E=2.1e-19 PF12697: Abhydrolase_6" amino acids 31 to 295 (265 residues), 61.4 bits, see alignment E=4.1e-20 PF00326: Peptidase_S9" amino acids 228 to 306 (79 residues), 33.4 bits, see alignment E=6.8e-12

Best Hits

KEGG orthology group: None (inferred from 99% identity to ppf:Pput_1338)

Predicted SEED Role

"Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative" (EC 3.1.1.23, EC 3.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.23 or 3.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EB5 at UniProt or InterPro

Protein Sequence (314 amino acids)

>PP_4551 Hydrolase, alpha/beta fold family (Pseudomonas putida KT2440)
MPHDAFWLPASEHCSLYVHQWLPATPVKAVVLLAHGMAEHAGRYQRLGQALSEAGFALFA
ADQRGHGRTAELGNLGLFARHHGWNAVVNDLGCVAQHIGQQFPCTPLFLFGHSMGSYIAQ
AYLLHHSGSLHGAILSGSNYQPAVLYRFARLIARLESWRQGPLGKSALIEWLSFGSFNNA
FKPNRTAFDWLSRDPGEVDQYVNDPLCGFRCTNQLWLDLLQGLAQISQPGNLAQIDPNLP
MLVIGGECDPVSAGKRLTHLADALRATGNRHVQLRVYPEARHEVLNETNRGEVIADILGW
LEQALALGRPARSE