Protein Info for PP_4533 in Pseudomonas putida KT2440

Annotation: mechanosensitive channel of miniconductance

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 22 to 47 (26 residues), see Phobius details amino acids 71 to 88 (18 residues), see Phobius details amino acids 102 to 126 (25 residues), see Phobius details amino acids 146 to 164 (19 residues), see Phobius details amino acids 170 to 186 (17 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 188 to 256 (69 residues), 54.6 bits, see alignment E=9.5e-19 PF21082: MS_channel_3rd" amino acids 339 to 398 (60 residues), 24.5 bits, see alignment E=3e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4533)

Predicted SEED Role

"Small-conductance mechanosensitive channel"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88ED2 at UniProt or InterPro

Protein Sequence (433 amino acids)

>PP_4533 mechanosensitive channel of miniconductance (Pseudomonas putida KT2440)
MDIQRIWRDSLDLWGTLDQHPMLHAAIGLAVLLLISLVVGRLARFLILHGARLLARQPAL
KWLDDLRHNKVFHRLAQTTPSLVLQFGLKLVPELSDTAQHFLGNVALAFTLLFMTMALSC
LLDALLDIYARTEHARTRSIKGYVQLAKMMLWIFASIIIVATLIDRSPLLLLSGLGAMSA
VLLLVYKDTLLSFVASVQLTSNDMLHVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNYRGMQQSGGRRIKRSLFIDAAGVRFLTREEEQRLSQVQLLSDYLAG
KRQELQNWNEALGPVADLSANRRKLTNIGTFRAFALAYLKNHPNVHPNMTCMVRQMQTTA
EGVPLEIYCFTTTTVWADYERIQGDIFDYLLAVLPEFGLSLYQQPSGNDMRVGLAGRTAA
EPMPRHLEEMSEA