Protein Info for PP_4499 in Pseudomonas putida KT2440

Annotation: putative maturation enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 TIGR00057: tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family" amino acids 19 to 212 (194 residues), 210 bits, see alignment E=1.1e-66 PF01300: Sua5_yciO_yrdC" amino acids 34 to 210 (177 residues), 166.5 bits, see alignment E=2.3e-53

Best Hits

Swiss-Prot: 61% identical to YCIO_ECOLI: Uncharacterized protein YciO (yciO) from Escherichia coli (strain K12)

KEGG orthology group: K07566, putative translation factor (inferred from 98% identity to ppg:PputGB1_4005)

Predicted SEED Role

"Hypothetical YciO protein, TsaC/YrdC paralog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EG5 at UniProt or InterPro

Protein Sequence (221 amino acids)

>PP_4499 putative maturation enzyme (Pseudomonas putida KT2440)
MNSLWRYEQDDYVSQFFQIHPENPQARLIKQAVEIIRKGGVVVYPTDSAYALGCQIGDKS
AIERVRRLRGLDKTHNFTLMCCDMSQLGLYAKVDTGTFRLLKAHVPGPYTFILNGTREVP
RLLLHDKRRTIGLRVPDHAITLALLAELGEPLMSVSLILPGDSEPMTDPYEIRERLEHHV
DLVIDGGFGDLKASTIIDLSGDEPELIREGCGDPTPFLVNA