Protein Info for PP_4492 in Pseudomonas putida KT2440

Annotation: carbohydrate efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 transmembrane" amino acids 9 to 32 (24 residues), see Phobius details amino acids 44 to 63 (20 residues), see Phobius details amino acids 82 to 107 (26 residues), see Phobius details amino acids 114 to 136 (23 residues), see Phobius details amino acids 148 to 168 (21 residues), see Phobius details amino acids 174 to 195 (22 residues), see Phobius details amino acids 216 to 241 (26 residues), see Phobius details amino acids 247 to 267 (21 residues), see Phobius details amino acids 279 to 297 (19 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details amino acids 336 to 359 (24 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details PF07690: MFS_1" amino acids 19 to 282 (264 residues), 55 bits, see alignment E=3.4e-19 amino acids 223 to 396 (174 residues), 47.1 bits, see alignment E=8.4e-17

Best Hits

Swiss-Prot: 71% identical to Y3873_PSEAB: Uncharacterized MFS-type transporter PA14_38730 (PA14_38730) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4492)

Predicted SEED Role

"Xanthine transporter,putative" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EH1 at UniProt or InterPro

Protein Sequence (400 amino acids)

>PP_4492 carbohydrate efflux transporter (Pseudomonas putida KT2440)
MPPRSPTSLAVTGQVVSIVLFTFIGYLNIGIPLAVLPGYVHNDLGFSAVIAGLVISVQYL
ATLLSRPGASRIIDNHGSKKAVMYGLAGCGLSGVFMLACAFLTHLPWLSLACLLVGRLVL
GSAESLVGSGAIGWGIGRVGAANTAKVISWNGIASYGALAIGAPLGVLMVKGIGLWSMGV
SIILLCVLGLLLAWPKQAAPIVSGVRLPFLRVLGKVFPHGSGLALGSIGFGTIATFITLY
YASRGWANAALTLSLFGASFISARLLFGNLINRIGGFRVAIACLSVETLGLLMLWLAPNA
ELALAGAALSGFGFSLVFPALGVEAVNQVSAANRGAAVGAYSLFIDLSLGVTGPLVGAVA
AGFGFASMFLFAAAAAACGLVLSLYLYRQGRRDRDRTTEA