Protein Info for PP_4481 in Pseudomonas putida KT2440

Annotation: Succinylornithine transaminase/acetylornithine aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03246: succinylornithine transaminase family
  • amino acids 8 to 403 (396 residues), 753.8 bits, E=4.1e-231
• TIGR00707: transaminase, acetylornithine/succinylornithine family
  • amino acids 17 to 400 (384 residues), 526.1 bits, E=4.5e-162
• PF00202: Aminotran_3
  • amino acids 20 to 400 (381 residues), 451.4 bits, E=2.6e-139
• PF00155: Aminotran_1_2
  • amino acids 171 to 347 (177 residues), 23.7 bits, E=2.6e-09

Best Hits

Swiss-Prot: 100% identical to ARGD_PSEPK: Acetylornithine aminotransferase (argD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 100% identity to ppf:Pput_1434)

MetaCyc: 80% identical to succinylornithine transaminase subunit (Pseudomonas aeruginosa)
Succinylornithine transaminase. [EC: 2.6.1.81]; Ornithine aminotransferase. [EC: 2.6.1.81, 2.6.1.13]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.13, 2.6.1.11]

Predicted SEED Role

"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-arginine degradation II (AST pathway) (5/5 steps found)
• L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-arginine degradation VI (arginase 2 pathway) (3/4 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)
• L-arginine degradation I (arginase pathway) (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.17

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.13 or 2.6.1.17 or 2.6.1.81

Sequence Analysis Tools

See P59319 at UniProt or InterPro

Protein Sequence (406 amino acids)

>PP_4481 Succinylornithine transaminase/acetylornithine aminotransferase (Pseudomonas putida KT2440)
MSVEQAPVQRADFDQVMVPNYSPAAFIPVRGEGSRVWDQSGRELIDFAGGIAVNALGHCH
PALVKALTEQANTLWHVSNVFTNEPALRLAHKLVDATFADRAFFCNSGAESNEAAFKLAR
RVAHDRFGPQKHEIIATVNSFHGRTLFTVSVGGQPKYSDGFGPKITGISHVPYNDLEALK
AQISDKTCAVVIEPIQGESGVVPADKAYLEGARKLCDEHNALLIFDEVQTGVGRTGSLYA
YQHYGVIPDILTSAKSLGGGFPIGAMLTTTELAKHLAVGTHGTTYGGNPLGCAVACAVLD
VVNTPETLAGIKAKHERFKTRLEQIGQQYNLFSQVRGVGLLLGCVLTEAWKGKAKDVLNA
AEKEGVMVLQAGPDVVRFAPSLVVEDADIDEGLDRFERAVATLTKG