Protein Info for PP_4469 in Pseudomonas putida KT2440

Annotation: ribose 1,5-bisphosphate phosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02322: phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN
  • amino acids 15 to 192 (178 residues), 230.4 bits, E=5.9e-73

Best Hits

Swiss-Prot: 66% identical to PHNN_PSEA7: Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K05774, ribose 1,5-bisphosphokinase [EC: 2.7.4.23] (inferred from 100% identity to ppu:PP_4469)

MetaCyc: 42% identical to ribose 1,5-bisphosphate phosphokinase (Sinorhizobium meliloti 1021)
Ribose 1,5-bisphosphate phosphokinase. [EC: 2.7.4.23]

Predicted SEED Role

"ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8)" (EC 2.7.4.8)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• dZTP biosynthesis (3/5 steps found)
• glyphosate degradation III (3/7 steps found)
• methylphosphonate degradation I (1/5 steps found)
• (aminomethyl)phosphonate degradation (3/8 steps found)

KEGG Metabolic Maps

• Pentose phosphate pathway
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.8

Use Curated BLAST to search for 2.7.4.23 or 2.7.4.8

Sequence Analysis Tools

See Q88EJ3 at UniProt or InterPro

Protein Sequence (199 amino acids)

>PP_4469 ribose 1,5-bisphosphate phosphokinase (Pseudomonas putida KT2440)
MAMQHDASDRRSGTGRLIFLVGPSGSGKDSLIDASREQLAAAGVEIARRVITRSAEAKGE
AAQGVTPEHFDTLRAQGAFAMHWRANGLDYGIPRQVDQWLAAGRAVLVNGSRAYLPEARQ
RYPDLLAVLVEVKPEVLRQRLLARGRESAEEVEQRLARSARLQAAADPSVHVLDNSSTLE
AAVAAFVSLLRDQGVLPRG