Protein Info for PP_4461 in Pseudomonas putida KT2440

Annotation: Major facilitator family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 55 to 73 (19 residues), see Phobius details amino acids 85 to 103 (19 residues), see Phobius details amino acids 110 to 130 (21 residues), see Phobius details amino acids 154 to 178 (25 residues), see Phobius details amino acids 185 to 206 (22 residues), see Phobius details amino acids 230 to 254 (25 residues), see Phobius details amino acids 270 to 290 (21 residues), see Phobius details amino acids 299 to 317 (19 residues), see Phobius details amino acids 324 to 343 (20 residues), see Phobius details amino acids 363 to 385 (23 residues), see Phobius details amino acids 391 to 412 (22 residues), see Phobius details PF00083: Sugar_tr" amino acids 17 to 220 (204 residues), 88.3 bits, see alignment E=5.3e-29 amino acids 243 to 422 (180 residues), 32.8 bits, see alignment E=3.7e-12 PF07690: MFS_1" amino acids 32 to 271 (240 residues), 68.6 bits, see alignment E=4.7e-23 amino acids 235 to 425 (191 residues), 44.5 bits, see alignment E=1e-15

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4461)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EK1 at UniProt or InterPro

Protein Sequence (430 amino acids)

>PP_4461 Major facilitator family transporter (Pseudomonas putida KT2440)
MTKTISTRKTWFAVATSGSGNLLEVYDFAVYAFFATTIAKLFFPNVDETTSLLQTFAAYG
AGFIARPLGSYVIGRIGDKRGRKPAMLLTIICMAIGSIGIGLIPTYETIGVGAPILLVML
RCLQGFAAGGEWGTSASYIVEWSPAGRKGFFGSFQSVSSSGGALLASLVASALLLIPAED
LLDWGWRVPFIAGGLAIFAFSLFLRAHAEETPEYVNSKAEVVNPTDSKPYVLGLQAFGFT
IFWTTLSYLVSAYMVTYTQNHAGLTRTEALISSNIALLLQITLIPVAGALSDRFGRKPLL
LLACLGTATLAYPILNLMSGGASFHQVVMLQCCLSALFAMYSGPGPATICEIFPTRLRNT
WMTVGYTLAVCCFGGFAPLISTWLISVTKIAASPAFLLIPAAVVSALVILKLPSTRISGF
KSAAKAEASY