Protein Info for PP_4456 in Pseudomonas putida KT2440

Annotation: opine oxidase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01946: Thi4" amino acids 5 to 45 (41 residues), 35.3 bits, see alignment 3.9e-12 PF01494: FAD_binding_3" amino acids 5 to 38 (34 residues), 32.3 bits, see alignment 3.2e-11 PF07992: Pyr_redox_2" amino acids 5 to 314 (310 residues), 79.8 bits, see alignment E=1.2e-25 PF00890: FAD_binding_2" amino acids 6 to 43 (38 residues), 31.7 bits, see alignment 5e-11 PF12831: FAD_oxidored" amino acids 6 to 85 (80 residues), 37.4 bits, see alignment E=9.8e-13 PF01266: DAO" amino acids 6 to 54 (49 residues), 34.3 bits, see alignment 9.8e-12 PF13450: NAD_binding_8" amino acids 9 to 43 (35 residues), 34 bits, see alignment 1.5e-11 PF04324: Fer2_BFD" amino acids 378 to 429 (52 residues), 35.3 bits, see alignment 6e-12 PF17806: SO_alpha_A3" amino acids 379 to 457 (79 residues), 46.7 bits, see alignment E=1.6e-15

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4456)

Predicted SEED Role

"D-nopaline/octopine oxidase subunit A @ Opine oxidase subunit A"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EK6 at UniProt or InterPro

Protein Sequence (464 amino acids)

>PP_4456 opine oxidase subunit A (Pseudomonas putida KT2440)
MSRTFDVVIVGAGPAGMSAAVVLSEQGLQVLVVDEQPAPGGQIWRAVETIAHTATGDILG
AEYKSGAELVQRFRACGAKYEPNTQMWKIEPGWTVFIKSNGVAEAVGARQVLLATGAQER
PAPFPGWTLPGVLTVGAAQILLKTSRQIPAEPVWVVGSGPLPLLYMAQLIRAGGKVAGWL
DTTPPGGWRRALPWASSMVAEFKEVSKGLAWLHEIRRSGARRIKGVKELRALGDGRLEQL
QFRLKSGELCTVPASVLLSHEGVIPSIHITQSLGCKHSWSAQQRCLVPDLDEWGETDQSG
IFVAGDGAGIGGAKAACVRGELAAIGMAKRAGRMAADEVAKNTDALSRRLQTLLRLRPML
DSMYPPRESIYTPSDETVVCRCEELTAGDIRKACAIAQPGVNQLKAFTRAGMGPCQGRQC
GYTIASIVAAEQQKAVEDVGFYRIRPPLKPITLGELASLDVEEK