Protein Info for PP_4454 in Pseudomonas putida KT2440

Annotation: putative Opine ABC transporter, permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 290 transmembrane" amino acids 24 to 46 (23 residues), see Phobius details amino acids 87 to 114 (28 residues), see Phobius details amino acids 122 to 145 (24 residues), see Phobius details amino acids 150 to 167 (18 residues), see Phobius details amino acids 198 to 218 (21 residues), see Phobius details amino acids 252 to 274 (23 residues), see Phobius details PF12911: OppC_N" amino acids 16 to 62 (47 residues), 52.8 bits, see alignment 2.9e-18 PF00528: BPD_transp_1" amino acids 103 to 286 (184 residues), 99.2 bits, see alignment E=2.5e-32

Best Hits

Swiss-Prot: 42% identical to DPPC_BACPE: Dipeptide transport system permease protein DppC (dppC) from Bacillus pseudofirmus (strain OF4)

KEGG orthology group: K02034, peptide/nickel transport system permease protein (inferred from 100% identity to ppu:PP_4454)

MetaCyc: 41% identical to dipeptide ABC transporter membrane subunit DppC (Escherichia coli K-12 substr. MG1655)
ABC-8-RXN [EC: 7.4.2.9]

Predicted SEED Role

"opine ABC transporter, permease protein, putative"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EK8 at UniProt or InterPro

Protein Sequence (290 amino acids)

>PP_4454 putative Opine ABC transporter, permease protein (Pseudomonas putida KT2440)
MTTLTAPLVSKPTTLWQALFQNRLSWIGIGLLVMIVLVALFAPLIAPYDPLQQNIAYRLE
PPSAEFWLGTDTYGRDVLSRLIYGTRVSLLVGFVAVMIAMVVGSALGVIAGYVGGITDRL
ITGFVDVLLSFPTLLLGLMVAAMLGASLENLIIAIAITEIAPFVRIARAPTIALKQRDFV
EAGRSLGYGPGRLMTVHILPNMISEVLVLGALWMATAIRTEASLSFIGLGVPPPAPTWGS
MIREGFENILDAWWLTVFPSLAILLTVLALSLLGDALRDANDPKTRSERS