Protein Info for PP_4434 in Pseudomonas putida KT2440

Annotation: D-amino acid dehydrogenase 1 small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF01266: DAO" amino acids 2 to 398 (397 residues), 268 bits, see alignment E=4.4e-83 PF13450: NAD_binding_8" amino acids 5 to 40 (36 residues), 27.9 bits, see alignment 5.8e-10

Best Hits

Swiss-Prot: 100% identical to DADA1_PSEPK: D-amino acid dehydrogenase 1 (dadA1) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to ppu:PP_4434)

MetaCyc: 61% identical to D-amino acid dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-11193 [EC: 1.4.5.1]; 1.4.5.- [EC: 1.4.5.1]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.5.1 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EM0 at UniProt or InterPro

Protein Sequence (432 amino acids)

>PP_4434 D-amino acid dehydrogenase 1 small subunit (Pseudomonas putida KT2440)
MRVLILGSGVVGTVSAYYLAREGFEVVVVDRQDGPAMETSFANAGQVSPGYASPWSAPGV
PLKAIRWMLQQHAPLAIKPTSSLDQYRWMIQMLRNCNPASYAVNKERMVRISEYSRDCLD
ELRAETGISYEARQLGTTQLFRTQAQVDNAAKDIAILKQSGVPFELLDRDGIAKAEPALA
SVRDKLAGALRLPNDQTGDCQMFTRKLAKMAEELGVEFRFGCNIDRLECSGDTISGVWID
GKLEVADQYVLALGSYSPHMLKPLGIQAPIYPLKGYSLTIPITDASMAPSSTILDETYKV
AITRFDDRIRVGGMAEIAGFDLSLNARRRDTLELVVGDLYPKGGDLTKATFWTGLRPATP
DGTPIVGKTKYRNLFLNTGHGTLGWTMSCGSGRLLADLMAGKKPKISAKGLDISRYSNQK
EAHNHGNPATAL